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莊秀美 博士
電子郵件: smchuang@dragon.nchu.edu.tw
實驗室位置: 生科大樓11樓1110實驗室 　　
實驗室電話:(04)2284-0896 分機 117
辦公室電話: (04)2284-0896 分機 116
傳真:

簡要經歷
國立中興大學生物醫學研究所助理教授 (2005.8~to date)
美國University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School 生物化學研究所博士後研究員 (2002.9~2005.6)
國立清華大學生命科學系博士後研究員( 2000.5~2002.9 )
工業技術研究院化學工業研究所副研究員 (1991.8~1995.7)

開設課程
高等細胞生物學(合開，課程召集人)
基因毒理與核酸修補
訊號傳遞與細胞週期調控
蛋白質降解途徑特論
Ubiquitination與人類疾病特論
活體內蛋白質功能分析
專題研究
文獻探討

研究生現況
碩士生
畢業生 : 郭柏竹
二年級 : 李彥德(生科系)、陳柏蓉
一年級 : 林冠廷

博士班
博一 : 吳宜玲


後排依序為 : 李彥德、吳宜玲、陳柏蓉、林冠廷

實驗室位置
國立中興大學生科大樓11F


實驗室設備


實驗室景觀


研究方向
蛋白質轉譯後修飾與降解機制
細胞訊息傳遞與ubiquitination的調控

研究主題
本實驗室以探討訊號傳遞在蛋白質轉譯後修飾與降解機制為主要的研究方向。蛋白質Rad23 N端含有一個類似UbL 區域，可以和proteasome結合。另外在C端含有二個UBA區域可和ubiquitin及連結上ubiquitin的蛋白質結合。Rad23在ubiquitin 媒介的蛋白質降解功能上，在演化上自酵母菌至人類是相當保留的。但是細胞內的訊號傳遞路徑如何調節Rad23的功能，以及它的蛋白質降解功能在核酸修補所扮演的角色，至今仍不瞭解。初步結果顯示，激酶Kin28可將Rad23磷酸化。磷32標定的胺基酸薄層液相層析顯示Kin28是在Ser, Thr及Tyr上將Rad23磷酸化。二維電泳及免疫分析顯示至少在體內有5-6 個位置會被磷酸化，而且Rad23 磷酸化會被生長階段及細胞週期所調控。本研究室將針對特定位置的胺基酸進行置換，以期更深入探討Rad23 磷酸化在核酸修補、蛋白質降解及細胞變異所扮演的角色。每一個帶有單一定位突變的蛋白質將會被分析其核酸修補、Rad4 結合能力、紫外線敏感性，以及蛋白質降解功能。更進一步地， 將分析磷酸化現象是否是細胞週期及外在逆境所調控。因Rad23 在核酸修補、細胞週期調控與蛋白質降解途徑扮演相當重要的角色，本研究可提供Rad23 在核酸修補與蛋白質降解二種功能之間的生化關係，及深入了解細胞如何調控Rad23 在核酸修補與ubiquitin 媒介的蛋白質降解。由於人類喪失Rad4-Rad23 複合物核酸修補功能會使皮膚細胞對紫外線敏感性大增進而導致皮膚癌。此研究對皮膚癌的成因與治療有進一步的貢獻。

近期參與之研究計劃
磷酸化調控Rad23在核酸修補與蛋白質降解途徑之機制研究 國科會。2006-2009
酵母菌蛋白Rad23被Kin28激酶磷酸化之研究。 國科會。2005-2006

期刊論文
PUBLICATIONS
(A) Published Papers in Refereed Journals
1. Chuang, S.-M., and Madura, K. (2005) Saccharomyces cerevisiae Ub-conjugating enzyme Ubc4 binds the proteasome in the presence of translationally-damaged proteins. Genetics 171, 1477-1484. (SCI)
2. Chuang, S.-M., Chen, L., Lambertson, D., Anand, M., Kinzy, T.G., and Madura, K. (2005) Proteasome-mediated degradation of co-translationally damaged proteins involves the translation elongation factor eEF1A. Mol. Cell. Biol. 25, 403-413. (SCI)
3. Lin, Y.-W., Chuang, S.-M., and Yang, J.-L. (2003) ERK1/2 Achieves Sustained Activation by Stimulating MAPK Phosphatase-1 Degradation via the Ubiquitin-Proteasome Pathway. J. Biol. Chem. 278, 21534-21541. (SCI)
4. Lin, Y.-W., Chuang, S.-M., and Yang, J.-L. (2003) Persistent activation of ERK1/2 by lead acetate increases nucleotide excision repair synthesis and confers anti-cytotoxicity and anti-mutagenicity. Carcinogenesis, 24, 53-61. (SCI)
5. Chuang, S.-M., Wang, I.-C., Hwua, Y.-S., and Yang, J.-L. (2003) Short-term depletion of catalase suppresses cadmium-elicited c-Jun N-terminal kinase activation and apoptosis: role of protein phosphatases. Carcinogenesis, 24, 7-15. (SCI)
6. Chuang, S.-M., and Yang, J.-L. (2001) Comparison of roles of three mitogen-activated protein kinases induced by chromium(VI) and cadmium in non-small-cell lung carcinoma cells. Molecular and Cellular Biochemistry, 222, 85-95. (SCI)
7. Chuang, S.-M., Liou, G.-Y., and Yang, J.-L. (2000) Activation of JNK, p38 and ERK mitogen-activated protein kinases by chromium(VI) is mediated through oxidative stress but does not affect cytotoxicity. Carcinogenesis, 21, 1491-1500. (SCI)
8. Chuang, S.-M., Wang, I.-C., and Yang, J.-L. (2000) Roles of JNK, p38 and ERK mitogen-activated protein kinases in the growth inhibition and apoptosis induced by cadmium. Carcinogenesis, 21, 1423-1432. (SCI)
9. Chang, M.C., Chang, S.Y., Chen, S.L., and Chuang, S.-M. (1992) Cloning and expression in Escherichia coli of the gene encoding an extracellular deoxyribonuclease (DNase) from Aeromonas hydrophilia. Gene, 122, 175-180. (SCI)

(B) Published abstracts in international meetings
1. Huang, S., Chuang, S.-M., Huang, Y-S., and Cheuh, P-J. (2007) Investigation of biological effects of TiO2 nanparticles on mammalian NIH3T3 cells. 3rd International Symposium on Nantechnology, Occupational and Environmental Health. Taipei, Taiwan, Aug. 29 - Sep 1, 2007.
2. Liu, S., Chuang, S.-M., Cheuh, P-J. (2006) The Biological function of tNOX in cell migration by RNAi-mediated gene silencing. The American Society for Cell Bilology, 46th Annual Meeting. San Diego, USA, December 9-13, 2006.
3. Madura, K., Lambertson, D., Chuang, S.-M., Ortolan, T., Chen, L., and Romero-Perez, L. (2004) Genetic studies reveal insight into Rad23 function. FASEB journal 18(8): C309-C309 Suppl. S, May 14, 2004.
4. Chuang, S.-M., and Yang, J.-L. (2001) Involvement of catalase in regulation of c-JUN N-terminal kinase activation. Mutation Research 483 (Suppl. 1) S139. (8th International Conference on Environmental Mutagens. Shizuoka, Japan, October 21-26, 2001.
5. Yang, J.-L., Liou, G.-Y., Chuang, S.-M., and Lin, Y.-W. (2001) Roles of three mitogen-activated protein kinases in UV-induced mutagenesis. Mutation Research 483 (Suppl. 1) S89. (8th International Conference on Environmental Mutagens. Shizuoka, Japan, October 21-26, 2001)
6. Chuang, S.-M., Yang J.-L. (1998) Roles of reactive oxygen species in the activation of JNK, p38, and ERK by chromium(VI) in non-small-cell lung carcinoma cells. Cancer Detection and Prevention 22 (Suppl. 1), Page S-60 (4th International Symposium on Predictive Oncology and Therapy. Nice, France, October 24-27, 1998)
7. Chuang, S.-M., Wang, I.-C, Hwua, Y.-S., Yang, J.-L. (1997) Role of JNK activation in genotoxicity induced by cadmium chloride in mammalian cells. Page 34. (American Cancer Society 8th Annual Research Conference. East Lansing, Michigan, September 12-13, 1997)